Kottke, I. & Suarez, J.P. (2006): <b>Tulasnellales from orchid mycorrhizas.</b> <i>Available online (http://www.tropicalmountainforest.org/data_pre.do?citid=584) from DFG-FOR816dw.</i>
Mycorrhizal state of epiphytic orchids was controversially discussed and state and mycobionts of the pleurothallidin orchids, occurring abundantly and with a high number of species on stems of trees in the Andean cloud forest, were unknown. Root samples of 77 adult individuals on total of the epiphytic orchids Stelis hallii, Stelis superbiens, Pleurothallis lilijae, and Stelis concinna were collected in a tropical mountain rain forest of southern Ecuador. Ultrastructural evidence of symbiotic interaction was combined with molecular sequencing of fungi directly from the mycorrhizas and isolation of mycobionts. Ultrastructural analyses displayed vital orchid mycorrhizas formed by fungi with an imperforate parenthesome and cell wall slime bodies typical for the genus Tulasnella. Three different Tulasnella isolates were obtained in pure culture. Phylogenetic analysis of the mycorrhiza forming Tulasnella and the Tulasnella isolates based on coding regions of the ribosomal large subunit (nucLSU) and the 5.8S subunit, including parts of the internal transcribed spacers, yielded seven distinct Tulasnella clades. Tulasnella mycobionts in Stelis concinna were restricted to two Tulasnella sequence types while the other orchids were associated with up to six Tulasnella sequence types. All Tulasnella sequences were new to science and distinct from known sequences of mycobionts of terrestrial orchids. These results indicate that tulasnelloid fungi, adapted to the conditions on tree stems, might be important for orchid growth and maintenance in the Andean cloud forest.
Additional Infos:
Technical details published in Mycological Research 110: 1257-1270, 2006, Diverse tulasnelloid fungi form mycorrhizas with epiphytic orchids in an Andean cloud forest.<br/>
Juan Pablo Suáreza, Michael Weiss, Andrea Abele, Sigisfredo Garnica, Franz Oberwinkler and Ingrid Kottke<br/>
Keywords:
| biodiversity | fungi |
Associated entities to this dataset:
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1
. data table entity --------
Table Name:
Tulasnellales
Tech. Details ...
Physical Structure Description:
Number of Records:
132
Attribute(s) ...
Attribute Name -- Attribute Label
Unit
Attribute definition
Organism
--
Organism
no unit
Organism
Method
(Sampling/processing description of the values of
this attribute by one ore more steps.)
--- Step
1
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Title
:
phylogenetic analysis
Description
:
phylogenetic analysis
GenBNoOrg
--
GenBank number of the organism
no unit
Accession number of the organism sequence in GenBank
Method
(Sampling/processing description of the values of
this attribute by one ore more steps.)
--- Step
1
---
Title
:
ID supplied by GenBank
Description
:
ID supplied by GenBank
SeqType
--
Sequence type of organism
no unit
Sequence type of organism
Method
(Sampling/processing description of the values of
this attribute by one ore more steps.)
--- Step
1
---
Title
:
DNA region
Description
:
DNA region
LifeForm
--
Life form
no unit
Description of life form
Method
(Sampling/processing description of the values of
this attribute by one ore more steps.)
--- Step
1
---
Title
:
light microscopic analysis of the fungal structures in the root (arbuscules, vesicles, hyphae)
Description
:
light microscopic analysis of the fungal structures in the root (arbuscules, vesicles, hyphae)
HostSpecies
--
Species name of the host
no unit
Species name of an host organism
Method
(Sampling/processing description of the values of
this attribute by one ore more steps.)
--- Step
1
---
Title
:
naming by the sampler
Description
:
naming by the sampler
SampleID
--
ID of the sample
no unit
ID of the sample
Method
(Sampling/processing description of the values of
this attribute by one ore more steps.)
--- Step
1
---
Title
:
naming by the sampler
Description
:
naming by the sampler
HostLifeForm
--
Life form of host
no unit
Life form of the host
Method
(Sampling/processing description of the values of
this attribute by one ore more steps.)
--- Step
1
---
Title
:
naming by the sampler
Description
:
naming by the sampler
PlotID
--
ID of the plot
no unit
ID of the plot
Method
(Sampling/processing description of the values of
this attribute by one ore more steps.)
--- Step
1
---
Title
:
naming by the members of the research group
Description
:
naming by the members of the research group
Site_name
--
Site name
no unit
Site name
Method
(Sampling/processing description of the values of
this attribute by one ore more steps.)