Download
Cite as:
Schlaeppi, K.; Bender, S.F.; Mascher, F.; Russo, G.; Patrignani, A.; Camenzind, T.; Hempel, S.; Rillig, M.C. &amp; van der Heijden, M.G. (2016): <b>High-resolution community profiling of arbuscular mycorrhizal fungi</b>. <i>New Phytologist</i> <b>xx</b>, xx.

Resource Description

Title: High-resolution community profiling of arbuscular mycorrhizal fungi
FOR816dw ID: 1493
Publication Date: 2016-07-06
License and Usage Rights: PAK 823-825 data user agreement. (www.tropicalmountainforest.org/dataagreementp3.do)
Resource Owner(s):
Individual: Klaus Schlaeppi
Contact:
Individual: S. Franz Bender
Contact:
Individual: Fabio Mascher
Contact:
Individual: Giancarlo Russo
Contact:
Individual: Andrea Patrignani
Contact:
Individual: Tessa Camenzind
Contact:
Individual: Stefan Hempel
Contact:
Individual: Matthias C. Rillig
Contact:
Individual: Marcel G.A. van der Heijden
Contact:
Abstract:
Summary<br/> Community analyses of arbuscular mycorrhizal fungi (AMF) using ribosomal small subunit<br/> (SSU) or internal transcribed spacer (ITS) DNA sequences often suffer from low resolution or<br/> coverage. We developed a novel sequencing based approach for a highly resolving and specific profiling of AMF communities.<br/> We took advantage of previously established AMF-specific PCR primers that amplify a<br/> c. 1.5-kb long fragment covering parts of SSU, ITS and parts of the large ribosomal subunit<br/> (LSU), and we sequenced the resulting amplicons with single molecule real-time (SMRT)<br/> sequencing.<br/> The method was applicable to soil and root samples, detected all major AMF families and<br/> successfully discriminated closely related AMF species, which would not be discernible using<br/> SSU sequences. In inoculation tests we could trace the introduced AMF inoculum at the<br/> molecular level. One of the introduced strains almost replaced the local strain(s), revealing<br/> that AMF inoculation can have a profound impact on the native community.<br/> The methodology presented offers researchers a powerful new tool for AMF community<br/> analysis because it unifies improved specificity and enhanced resolution, whereas the drawback of medium sequencing throughput appears of lesser importance for low-diversity groups<br/> such as AMF.
Keywords:
| arbuscular mycorrhiza | mycorrhiza |
Literature type specific fields:
ARTICLE
Journal: New Phytologist
Volume: xx
Page Range: xx
Metadata Provider:
Individual: Tessa Camenzind
Contact:
Online Distribution:
Download File: http://www.lcrs.de/publications.do?citid=1493


Quick search

  • Publications:
  • Datasets: