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Strutzenberger, P.; Brehm, G. &amp; Fiedler, K. (2011): <b>DNA barcoding-based species delimitation increases species count of Eois (Geometridae) moths in a well-studied tropical mountain forest by up to 50%</b>. <i>Insect Science</i> <b>18</b>, 349?362.

Resource Description

Title: DNA barcoding-based species delimitation increases species count of Eois (Geometridae) moths in a well-studied tropical mountain forest by up to 50%
Short Name: DNA barcoding of Eois moths
FOR816dw ID: 1033
Publication Date: 2011-06-01
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Resource Owner(s):
Individual: Patrick Strutzenberger
Contact:
Individual: Gunnar Brehm
Contact:
Individual: Konrad Fiedler
Contact:
Abstract:
The genus Eois comprises an important part of megadiverse assemblages of<br/> geometrid moths in mountain rainforests of southern Ecuador. In this study we report:<br/> (i) on the construction of a DNA barcode library of Eois for identification purposes;<br/> and (ii) the exploration of species diversity through species delimitation by pair-wise<br/> distance thresholds. COI barcode sequences were generated from 408 individuals (at<br/> least 105 species) collected on a narrow geographic scale (&#8764;40 km2) in the Reserva<br/> Biol´ogica San Francisco. Analyses of barcode sequence divergence showed that species<br/> delimitations based solely on external morphology result in broad overlap of intra- and<br/> interspecific distances. Species delimitation at a 2% pair-wise distance threshold reveals<br/> a clear barcoding gap. Fifty-two previously unrecognized species were identified, 31 of<br/> which could only be distinguished by an integrative taxonomy approach. Twelve additional<br/> putative species could only be recognized by threshold-based delimitation. Most splits<br/> resulted in two or three newly perceived cryptic taxa. The present study increased the<br/> number of Eois species recorded from that small area of Andean mountain forest from<br/> 102 to 154 (morphology- plus integrative taxonomy-based) or even 166 (sequence-based),<br/> leaving the species accumulation curve still far from reaching an asymptote. Notably, in<br/> no case did two or more previously distinguished morphospecies have to be lumped. This<br/> barcode inventory can be used to match larvae to known adult samples without rearing,<br/> and will therefore be of vital help to extend the currently limited knowledge about food<br/> plant relationships and host specialization.
Keywords:
| Ecuador | Larentiinae | cryptic species | integrative taxonomy | larvae |
Literature type specific fields:
ARTICLE
Journal: Insect Science
Volume: 18
Page Range: 349?362
Metadata Provider:
Individual: Bernhard Runzheimer
Contact:
Online Distribution:
Download File: http://www.lcrs.de/publications.do?citid=1033


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