Krüger, M.; Stockinger, H.; Krüger, C. & Schüßler, A. (2009): <b>DNA-based species-level detection of arbuscular mycorrhizal fungi: one PCR primer set for all AMF</b>. <i>New Phytologist</i> <b>183</b>, 212-223.
Resource Description
Title:
DNA-based species-level detection of arbuscular mycorrhizal fungi: one PCR primer set for all AMF
FOR816dw ID:
391
Publication Date:
2009-04-15
License and Usage Rights:
Resource Owner(s):
Individual:
Manuela Krüger
Contact:
email:
manuela.krueger <at> lrz.uni-muenchen.de
Grosshaderner Str. 4
82152 Planegg-Martinsried
Germany
email:
arthur.schuessler <at> lmu.de
Grosshaderner Str. 4
Genetics, Schuessler-Lab
Department Biology I, Biocenter
D-82152 Martinsried
D-82152 Martinsried
Germany
Abstract:
- Presently, molecular ecological studies of arbuscular mycorrhizal fungi (AMF) are only possible above species-level when targeting entire communities. To improve molecular species characterisation and to allow species-level community analyses in the field, a set of newly designed AMF specific PCR primers is successfully tested.<br/>
- Nuclear rDNA fragments from diverse phylogenetic AMF lineages were sequenced and analysed to design four primer mixtures, each targeting one binding site in the small (SSU) or large subunit rDNA (LSU). To allow species resolution, they span a fragment covering the partial SSU, whole internal transcribed spacer rDNA region (ITS), and partial LSU.<br/>
- The new primers are suitable to i) specifically amplify AMF rDNA from material that may be contaminated by other organisms, e.g., samples from pot cultures or the field, ii) characterise the diversity of AMF species from field samples, and iii) amplify a SSU-ITS-LSU fragment that allows phylogenetic analyses with species-level resolution.<br/>
- The described PCR primers can be used for monitoring of entire AMF field communities, based on a single rDNA marker region. Their application will improve the base for deep sequencing approaches, and moreover they can be efficiently used as DNA barcoding primers.
Literature type specific fields:
ARTICLE
Journal:
New Phytologist
Volume:
183
Page Range:
212-223
Publisher:
Blackwell Publishing
Metadata Provider:
Individual:
Arthur Schüßler
Contact:
email:
arthur.schuessler <at> lmu.de
Grosshaderner Str. 4
Genetics, Schuessler-Lab
Department Biology I, Biocenter
D-82152 Martinsried
D-82152 Martinsried
Germany
