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Herrera, P.; Suarez, J.P. &amp; Kottke, I. (2010): <b>Orchids keep the ascomycetes outside: a highly diverse group of ascomycetes colonizing the velamen of epiphytic orchids from a tropical mountain rainforest in Southern Ecuador</b>. <i>Mycology</i> <b>1</b>(4), 262-268.

Resource Description

Title: Orchids keep the ascomycetes outside: a highly diverse group of ascomycetes colonizing the velamen of epiphytic orchids from a tropical mountain rainforest in Southern Ecuador
FOR816dw ID: 1335
Publication Date: 2010-12-10
License and Usage Rights: PAK 823-825 data user agreement. (www.tropicalmountainforest.org/dataagreementp3.do)
Resource Owner(s):
Individual: Paulo Herrera
Contact:
Individual: Juan Pablo Suarez
Contact:
Individual: Ingrid Kottke
Contact:
Abstract:
Orchid mycorrhizal detection, based only on fungal isolation from roots, is biased due to difficulties in isolating the respec- tive fungi. Previous investigations have shown that mostly ascomycetes, housed in the sheltered compartment of the vela- men covering the roots of epiphytic orchids, are isolated on agar plates. Roots of 83 individual epiphytic orchid of Stelis hallii, S. superbiens, S. concinna, Stelis sp. and Pleurothallis lilijae were sampled in the Reserva Biológica San Francisco, Southern Ecuador. The velamen was partly removed and root tissue macerated and transferred to Petri dishes containing corn meal agar, malt extract agar and Melin Norkrans modified medium. DNA was extracted from the cultures and the 5.8S-ITS region and partial nrLSU sequenced. Phylogenetic analysis revealed members of 12 orders of ascomycetes associ- ated with the roots of these orchids, a much higher diversity than previously known. Twelve isolates were related to the Helotiales. Our results have uncovered some of the fungal diversity within the velamen of epiphytic orchids; fungi invading, most likely, from tree bark or humus accumulation close to the roots.
Keywords:
| neotropical mountain rain forest | ascomycota | epiphytic orchids | velamen | Helotiales |
Literature type specific fields:
ARTICLE
Journal: Mycology
Volume: 1
Issue: 4
Page Range: 262-268
Metadata Provider:
Individual: Ingeborg Haug
Contact:
Online Distribution:
Download File: http://www.tropicalmountainforest.org/publications.do?citid=1335


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