Rillig, M.C.; Camenzind, T.; Velescu, A.; Wilcke, W.; Homeier, J.; Horn, S. & Hempel, S. (2014): <b>Nitrogen and phosphorus additions impact arbuscular mycorrhizal abundance and molecular diversity in a tropical montane forest</b>. <i>Global Change Biology</i> <b>--</b>, --.
Resource Description
Title:
Nitrogen and phosphorus additions impact arbuscular mycorrhizal abundance and molecular diversity in a tropical montane forest
FOR816dw ID:
1267
Publication Date:
2014-06-14
License and Usage Rights:
PAK 823-825 data user agreement. (www.tropicalmountainforest.org/dataagreementp3.do)
Resource Owner(s):
Individual:
Matthias C. Rillig
Contact:
email:
rillig <at> zedat.fu-berlin.de
14195 Berlin
Germany
Individual:
Tessa Camenzind
Contact:
email:
tessac <at> zedat.fu-berlin.de
14195 Berlin
Germany
Individual:
Andre Velescu
Contact:
email:
andre.velescu <at> kit.edu
Karlsruher Institut für Technologie
Institut für Geographie und Geoökologie
Arbeitsgruppe Geomorphologie und Bodenkunde
Reinhard-Baumeister-Platz 1
76131 Karlsruhe
Germany
Individual:
Wolfgang Wilcke
Contact:
email:
wolfgang.wilcke <at> kit.edu
Karlsruhe Institute of Technology
Institute of Geography and Geoecology
Reinhard-Baumeister-Platz 1
76131 Karlsruhe
Baden-Württemberg
Germany
Individual:
Jürgen Homeier
Contact:
email:
jhomeie <at> gwdg.de
Faculty of Resource Management
University of Applied Sciences and Arts (HAWK)
37077 Göttingen
Germany
Individual:
Sebastian Horn
Contact:
email:
webmaster <at> tropicalmountainforest.org
Individual:
Stefan Hempel
Contact:
email:
webmaster <at> tropicalmountainforest.org
Abstract:
Increased nitrogen (N) depositions expected in the future endanger the diversity and stability of ecosystems primarily limited by N, but also often co-limited by other nutrients like phosphorus (P). In this context a nutrient manipulation experiment (NUMEX) was set up in a tropical montane rainforest in southern Ecuador, an area identified as biodiversity hotspot. We examined impacts of elevated N and P availability on arbuscular mycorrhizal fungi (AMF), a group of obligate biotrophic plant symbionts with an important role in soil nutrient cycles. We tested the hypothesis that increased nutrient availability will reduce AMF abundance, reduce species richness and shift the AMF community toward lineages previously shown to be favored by fertilized conditions. NUMEX was designed as a full factorial randomized block design. Soil cores were taken after 2 years of nutrient additions in plots located at 2000 m above sea level. Roots were extracted and intraradical AMF abundance determined microscopically; the AMF community was analyzed by 454-pyrosequencing targeting the large subunit rDNA. We identified 74 operational taxonomic units (OTUs) with a large proportion of Diversisporales. N additions provoked a significant decrease in intraradical abundance, whereas AMF richness was reduced significantly by N and P additions, with the strongest effect in the combined treatment (39% fewer OTUs), mainly influencing rare species. We identified a differential effect on phylogenetic groups, with Diversisporales richness mainly reduced by N additions in contrast to Glomerales highly significantly affected solely by P. Regarding AMF community structure, we observed a compositional shift when analyzing presence/absence data following P additions. In conclusion, N and P additions in this ecosystem affect AMF abundance, but especially AMF species richness; these changes might influence plant community composition and productivity and by that various ecosystem processes.
