Schlaeppi, K.; Bender, S.F.; Mascher, F.; Russo, G.; Patrignani, A.; Camenzind, T.; Hempel, S.; Rillig, M.C. & van der Heijden, M.G. (2016): <b>High-resolution community profiling of arbuscular mycorrhizal fungi</b>. <i>New Phytologist</i> <b>xx</b>, xx.
Resource Description
Title:
High-resolution community profiling of arbuscular mycorrhizal fungi
FOR816dw ID:
1493
Publication Date:
2016-07-06
License and Usage Rights:
PAK 823-825 data user agreement. (www.tropicalmountainforest.org/dataagreementp3.do)
Resource Owner(s):
Individual:
Klaus Schlaeppi
Contact:
email:
webmaster <at> tropicalmountainforest.org
Individual:
S. Franz Bender
Contact:
email:
webmaster <at> tropicalmountainforest.org
Individual:
Fabio Mascher
Contact:
email:
webmaster <at> tropicalmountainforest.org
Individual:
Giancarlo Russo
Contact:
email:
webmaster <at> tropicalmountainforest.org
Individual:
Andrea Patrignani
Contact:
email:
webmaster <at> tropicalmountainforest.org
Individual:
Tessa Camenzind
Contact:
email:
tessac <at> zedat.fu-berlin.de
14195 Berlin
Germany
Individual:
Stefan Hempel
Contact:
email:
webmaster <at> tropicalmountainforest.org
Individual:
Matthias C. Rillig
Contact:
email:
rillig <at> zedat.fu-berlin.de
14195 Berlin
Germany
Individual:
Marcel G.A. van der Heijden
Contact:
email:
webmaster <at> tropicalmountainforest.org
Abstract:
Summary<br/>
Community analyses of arbuscular mycorrhizal fungi (AMF) using ribosomal small subunit<br/>
(SSU) or internal transcribed spacer (ITS) DNA sequences often suffer from low resolution or<br/>
coverage. We developed a novel sequencing based approach for a highly resolving and specific profiling of AMF communities.<br/>
We took advantage of previously established AMF-specific PCR primers that amplify a<br/>
c. 1.5-kb long fragment covering parts of SSU, ITS and parts of the large ribosomal subunit<br/>
(LSU), and we sequenced the resulting amplicons with single molecule real-time (SMRT)<br/>
sequencing.<br/>
The method was applicable to soil and root samples, detected all major AMF families and<br/>
successfully discriminated closely related AMF species, which would not be discernible using<br/>
SSU sequences. In inoculation tests we could trace the introduced AMF inoculum at the<br/>
molecular level. One of the introduced strains almost replaced the local strain(s), revealing<br/>
that AMF inoculation can have a profound impact on the native community.<br/>
The methodology presented offers researchers a powerful new tool for AMF community<br/>
analysis because it unifies improved specificity and enhanced resolution, whereas the drawback of medium sequencing throughput appears of lesser importance for low-diversity groups<br/>
such as AMF.
Keywords:
| arbuscular mycorrhiza | mycorrhiza |
Literature type specific fields:
ARTICLE
Journal:
New Phytologist
Volume:
xx
Page Range:
xx
Metadata Provider:
Individual:
Tessa Camenzind
Contact:
email:
tessac <at> zedat.fu-berlin.de
14195 Berlin
Germany
