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Platform data

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Measurement of arbuscular mycorrhizal (AMF) abundance in the NUMEX plots at all elevational sites. AMF root colonization, hyphal length, spore counts and NLFA 16:1w5 marker in soil were determined. Additionally, we quantified non-mycorrhizal fungal root colonization and hyphal length.

Monthly measurement of soil temperature and soil gas fluxes (CO2, N2O) in the NUMEX plots in Bombuscaro, ECSF und Cajanuma

Monthly measurements of gas fluxes were performed using static vented chambers.
Soil temperature was taken simultaneously with gas measurements.

Measurements of gas flux (CO2, CH4, N2O) in canopy soils of NUMEX plots on four dates (Sept/Nov 2011 and Jan/April 2012). Measurements were taken in 3 of the 4 NUMEX blocks at each elevation; treatments measured included C, N, NP and P.

Gas fluxes were measured by incubating intact soil cores.
Gas samples were analyzed on a gas chromatograph with a FID and ECD.
Note that the units for CO2 are mg CO2-C m-2 h-1 (not g CO2-C).

Attributes measured and morphological attribute analyzed in 2012  after harvesting the seedling transplantation experiment using Pouteria torta species.

refer to  Cárate-Tandalla, D., Leuschner, C., Homeier, J. (2015).Performance of Seedlings of a Shade-Tolerant Tropical Tree Species after Moderate Addition of N and P - Frontiers in Earth Science. http://dx.doi.org/10.3389/feart.2015.00075

AMF root colonization of roots extracted from the upper soil layer (5cm) at all NUMEX plots. Roots are stained with Trypan Blue, AMF root colonization is examined by the line-intersect method.

Monthly time series of d13C and d15N isotope measurements in litterfall at NUMEX San Francisco between 2007 and 2009. Litterfall was collected on all 20 plots at NUMEX on 6 subplots per plot (A-F) with polyethylene mesh traps (0.6 x 0.6 m). On each plot, two of the six litterfall traps were randomly sampled monthly. The samples were dried at 40 °C for one week and stored at the ECSF until transport to Germany or Switzerland for chemical analyses. The two collected replicates were bulked per plot and collecting interval resulting in one litterfall sample per plot and month. Samples were ground to <50 µm with a planetary ball mill (Retsch PM200, Haan, Germany) prior to isotope measurements of d13C and d15N by EA/IRMS (ThermoFisher, Bremen, Germany)

For the seedling transplantation experiment conducted in NUMEX plots in Bombuscaro using as a target species Pouteria torta for monitoring during one year the effects of fertilization in a common species. This data set correspond to the initial measurements of the experiment

refers to Cárate-Tandalla, D., Leuschner, C., Homeier, J. (2015). Performance of Seedlings of a Shade-Tolerant Tropical Tree Species after Moderate Addition of N and P - Frontiers in Earth Science. http://dx.doi.org/10.3389/feart.2015.00075

Fortnightly time series of measured electrical conductivity and pH values in litter leachate at NUMEX San Francisco. The fortnightly time series extends between August 2007 and December 2016. Litter leachate was sampled on the 20 NUMEX plots with zero-tension lysimeters below the organic layer. On each plot 3 replicates were collected, volume-weighted and bulked to one sample per plot. Electrical conductivity and pH values were measured immediately in unfiltered aliquots in the laboratory of the ECSF.

Fortnightly time series of measured concentrations of Cl, PO4-P, Total P, NH4-N, NO3-N, Total N, K, Na, Mg, Ca and Total organic C in throughfall at NUMEX San Francisco. The fortnightly time series extends between August 2007 and March 2013. Throughfall was sampled on the 20 NUMEX plots with Hellmann-type collectors. On each plot 20 replicates were collected, volume-weighted and bulked to one sample per plot. Samples were immediately filtered (4-7 µm) in the laboratory of the ECSF  and stored at -20 °C until transport in frozen state to Germany or to Switzerland for concentration measurements.

Arbuscular mycorrhizal root colonization (%), colonized root length (cm/cm³) and fine root parameters in response to nutrient additions (N, P and NP) are reported. Samples were taken in October 2010 at the NUMEX plots at 2000m.

Operational taxonomic units (OTUs) of arbuscular mycorrhizal fungi (AMF) detected in roots sampled at the 2000m NUMEX sites in October 2010.

Soil data of NUMEX plots measured in 2013. Percentage of C and N, as well as soil pH were determined.

We evaluated the amount of waterstable soil aggregates at the Bombuscaro NUMEX plots. We included various potential soil parameters that are known to have an effect on the aggregation process.

Soil aggregation parameters were measured in NUMEX at 1000m in 2013. Water-stable soil aggregates were determined as well as the mean weight diameter of soil based on dry-sieving.