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Monthly time series of measured concentrations of Al, Cu, Fe, Mn, Zn, C, N, S, P, K, Na, Mg, Ca in litterfall at NUMEX San Francisco between 2007 and 2013. Litterfall was collected on all 20 plots at NUMEX on 6 subplots per plot (A-F) with polyethylene mesh traps (0.6 x 0.6 m). On each plot, two of the six litterfall traps were randomly sampled monthly. The samples were dried at 40 °C for one week and stored at the ECSF until transport to Germany or Switzerland for chemical analyses. The two collected replicates were bulked per plot and collecting interval resulting in one litterfall sample per plot and month. Samples were ground to <50 µm with a planetary ball mill (Retsch PM200, Haan, Germany) prior to elemental analysis of C, N, and S by thermal combustion (Elementar Analysensysteme, Hanau, Germany) and acid microwave digestion with concentrated HNO3. Element concentrations in the digested samples were measured by AAS, ICP-OES or ICP-MS.

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Fortnightly time series of measured electrical conductivity and pH values in throughfall at NUMEX San Francisco. The fortnightly time series extends between August 2007 and December 2016. Throughfall was sampled on the 20 NUMEX plots with Hellmann-type collectors. On each plot 20 replicates were collected, volume-weighted and bulked to one sample per plot. Electrical conductivity and pH values were measured immediately in unfiltered aliquots in the laboratory of the ECSF.

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Fortnightly time series of measured electrical conductivity and pH values in soil solution (30 cm mineral soil depth) at NUMEX San Francisco. The fortnightly time series extends between August 2007 and December 2016. Soil solution was sampled on the 20 NUMEX plots with ceramic suction cups having a pore size of 1 µm installed at 30 cm depth in the mineral soil. On each plot 3 replicates were collected and bulked to one sample per plot. Electrical conductivity and pH values were measured immediately in unfiltered aliquots in the laboratory of the ECSF.

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Measurement of arbuscular mycorrhizal (AMF) abundance in the NUMEX plots at all elevational sites. AMF root colonization, hyphal length, spore counts and NLFA 16:1w5 marker in soil were determined. Additionally, we quantified non-mycorrhizal fungal root colonization and hyphal length.

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AMF root colonization of roots extracted from the upper soil layer (5cm) at all NUMEX plots. Roots are stained with Trypan Blue, AMF root colonization is examined by the line-intersect method.

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Operational taxonomic units (OTUs) of arbuscular mycorrhizal fungi (AMF) detected in roots sampled at the 2000m NUMEX sites in October 2010.

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Roots were collected at all NUMEX Plots (ECSF) in January 2009, except Ca treatments. 3 subplots were sampled per plot. Roots were stained with Trypan Blue and the percentage of root colonization determined using the line-intersect method.

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Fortnightly time series of measured concentrations of Cl, PO4-P, Total P, NH4-N, NO3-N, Total N, K, Na, Mg, Ca and Total organic C in soil solution (15 cm mineral soil depth) at NUMEX San Francisco. The fortnightly time series extends between August 2007 and March 2013. Soil solution was sampled on the 20 NUMEX plots with ceramic suction cups having a pore size of 1 µm installed at 15 cm depth in the mineral soil. On each plot 3 replicates were collected and bulked to one sample per plot. The samples were stored at -20 °C until transport in frozen state to Germany or to Switzerland for concentration measurements.

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Changes in AMF root colonization following N and P additions at 2000m.