- Effects from climate change, adaptations and
greenhouse gas reduction at 2050

Abstract:

Shows how simulated biomass, soil moisture and greenhouse gas emissions are affected by the measured N deficit of the Giessen FACE grassland.

Abstract:

Global warming is currently a much discussed topic with as yet largely unexplored consequences for agro-ecosystems. Little is known about the warming effect on the bacterial microbiota inhabiting the plant surface (phyllosphere), which can have a strong impact on plant growth and health, as well as on plant diseases and colonization by human pathogens. The aim of this study was to investigate the effect of moderate surface warming on the diversity and composition of the bacterial leaf microbiota of the herbaceous plant Galium album. Leaves were collected from four control and four surface warmed (+2°C) plots located at the field site of the Environmental Monitoring and Climate Impact Research Station Linden in Germany over a 6-year period. Warming had no effect on the concentration of total number of cells attached to the leaf surface as counted by Sybr Green I staining after detachment, but changes in the diversity and phylogenetic composition of the bacterial leaf microbiota analyzed by bacterial 16S rRNA gene Illumina amplicon sequencing were observed. The bacterial phyllosphere microbiota were dominated by Proteobacteria, Bacteroidetes, and Actinobacteria. Warming caused a significant higher relative abundance of members of the Gammaproteobacteria, Actinobacteria, and Firmicutes, and a lower relative abundance of members of the Alphaproteobacteria and Bacteroidetes. Plant beneficial bacteria like Sphingomonas spp. and Rhizobium spp. occurred in significantly lower relative abundance in leaf samples of warmed plots. In contrast, several members of the Enterobacteriaceae, especially Enterobacter and Erwinia, and other potential plant or human pathogenic genera such as Acinetobacter and insect-associated Buchnera and Wolbachia spp. occurred in higher relative abundances in the phyllosphere samples from warmed plots. This study showed for the first time the long-term impact of moderate (+2°C) surface warming on the phyllosphere microbiota on plants. A reduction of beneficial bacteria and an enhancement of potential pathogenic bacteria in the phyllosphere of plants may indicate that this aspect of the ecosystem which has been largely neglected up till now, can be a potential risk for pathogen transmission in agro-ecosystems in the near future.

Abstract:

The increase in atmospheric greenhouse gas concentrations from anthropogenic activities is the major driver of recent global climate change1. The stimulation of plant photosynthesis due to rising atmospheric carbon dioxide concentrations ([CO2]) is widely assumed to increase the net primary productivity (NPP) of C3 plants—the CO2 fertilization effect (CFE). However, the magnitude and persistence of the CFE under future climates, including more frequent weather extremes, are controversial. Here we use data from 16 years of temperate grassland grown under ‘free-air carbon dioxide enrichment’ conditions to show that the CFE on above-ground biomass is strongest under local average environmental conditions. The observed CFE was reduced or disappeared under wetter, drier and/or hotter conditions when the forcing variable exceeded its intermediate regime. This is in contrast to predictions of an increased CO2 fertilization effect under drier and warmer conditions. Such extreme weather conditions are projected to occur more intensely and frequently under future climate scenarios. Consequently, current biogeochemical models might overestimate the future NPP sink capacity of temperate C3 grasslands and hence underestimate future atmospheric [CO2] increase.

Abstract:

Compares simulations of plant biomass and CO2 emissions of the Giessen FACE grassland with and without groundwater-borne nitrate.

Abstract:

The presentation of the Working package B2 at the general assembly of the FACE2FACE consortium at the 4th of July, 2017.

Abstract:

Overview of the model setup for the Giessen FACE grassland ecosystem, including preliminary simulation results for CO2 emissions and outlook.

Abstract:

The continuous increase in atmospheric carbon dioxide (CO2) contributes to changes in plant evapotranspiration and terrestrial water Budgets in two ways. Firstly, elevated CO2 can result in a water saving effect, since increasing CO2 reduces stomatal opening and therefore decreases transpiration. Secondly, CO2 fertilization increases biomass accumulation and leaf area at plant canopy Level, likely increasing plant transpiration. Vegetation and hydrological models can be used to investigate the CO2 Response and the bidirectional effects outlined above, including their relative contribution to the changes in the water cycle. However, the intrinsic plant-soil interaction and the uncertainty related to model parameterization have rarely been considered. Hence, we coupled a detailed plant growth and soil hydrological model by using the generic model frameworks Plant growth Modelling Framework (PMF) and Catchment Modelling Framework (CMF). Up to date response mechanisms have been implemented in PMF to simulate the various ways of how plant physiology is influenced by elevated CO2. Both models interact by using the Python computer language. Applying the coupled PMF-CMF model we investigate the effects of elevated CO2 in a number of plant physiological and environmental variables such as biomass, leaf area index and soil moisture using field data of a long-term Free Air Carbon dioxide Enrichment (FACE) Experiment in Giessen, Germany. In this Experiment, various grassland varieties (herbs, legumes, grasses) grow under elevated (+20%) and ambient CO2 since 1997. A Monte Carlo based uncertainty analysis (GLUE) is conducted to investigate the coupled PMF-CMF parameter space. The focus will be on the identification of parameters for plant and soil, which are the drivers for the CO2 Response of the terrestrial water balance. We will present first results of the simulation of biomass accumulation and transpiration under ambient and elevated CO2 concentrations.

Abstract:

Four yellow-pigmented, Gram-stain-negative, rod-shaped bacteria, strains PP-WC-4G-234T, PP-CE-2G-454T, PP-WC-1G-202 and PP-CC-3G-650, were isolated from the phyllosphere of Galium album. The strains shared 99.7–100?% 16S rRNA gene sequence similarity but could be differentiated by genomic fingerprinting using rep- and random amplification of polymorphic DNA PCRs. Phylogenetic analysis based on the 16S rRNA gene placed the strains within the family Aurantimonadaceae with highest 16S rRNA gene sequence similarity of 97.2–97.3?% to the type strain of Aureimonas phyllosphaerae. Sequence similarities to all other Aurantimonadaceae were below 97?%. The main cellular fatty acids of the strains were C18?:?1 ?7c as the predominant fatty acid followed by C16?:?0 and summed feature 3 (C16?:?1 ?7c/C16?:?1 ?8c). The polyamine patterns of strains PP-WC-4G-234T and PP-CE-2G-454T contained sym-homospermidine as a major compound, and the major respiratory quinone was ubiquinone Q-10. Predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine, sulfoquinovosyldiacylglycerol, three unidentified phospholipids and one unidentified lipid only detectable after total lipid staining. The DNA G+C content was 66.4, 68.9, 67.4 and 70.5 mol% for strains PP-WC-4G-234T, PP-CE-2G-454T, PP-WC-1G-202 and PP-CC-3G-650, respectively. Based on phylogenetic, chemotaxonomic and phenotypic analyses we propose two novel species of the genus Aureimonas, Aureimonas galii sp. nov. with PP-WC-4G-234T (=LMG 28655T=CIP 110892T) as the type strain and Aureimonas pseudogalii sp. nov. with PP-CE-2G-454T (=LMG 29411T=CCM 8665T) as the type strain and two further strains representing the same species, PP-WC-1G-202 and PP-CC-3G-650.

Abstract:

Overview of the B3 working package of the FACE2FACE Programme. Includes methods and model setup, N balance of the field site, simulation results of biomass and CO2 emissions with and without groundwater, as well as an outlook for the phase-out period.

Abstract:

Facing rising atmospheric CO2 concentrations, subsoils may play an important role in the global carbon (C) cycle due to the presence of unsaturated mineral surfaces. Further, macroaggregation is considered a crucial process influencing C sequestration. However, analyses on subsoil aggregation and C retention processes under long-term elevated CO2 (eCO2) are lacking. In this study we investigated the long-term effect of +20% above ambient CO2 concentration (corresponds to conditions reached 2035–2045) in a temperate grassland ecosystem at the Giessen Free Air CO2 Enrichment (Gi-FACE), Germany. A depth-dependent response of macroaggregation to eCO2 was observed: While in subsoil (15–45?cm depth) macroaggregation increased under eCO2, no CO2 induced change in macroaggregation was detected in topsoil (0–15?cm). Increased macroaggregation in subsoil coincided with higher SOC content of large macroaggregates (LM). Mean residence time (MRT) of SOC in aggregate-size classes were not different among each other under eCO2. However, macroaggregates and bulk soil differed in their MRT between soil depths. Despite increased macroaggregation and an estimated high SOC sequestration potential in subsoil we could not observe an increase in SOC content of bulk soil.

Abstract:

Rising atmospheric CO2 concentrations are expected to increase nitrous oxide (N2O) emissions from soils via changes in microbial nitrogen (N) transformations triggering a positive feedback reaction that could accelerate climate change. Several studies have shown N2O emission increases under elevated atmospheric CO2 (eCO2), but the underlying processes are not yet fully understood. Here we present results showing changes in soil N transformation dynamics from the Giessen Free Air CO2 Enrichment (GiFACE): a permanent grassland that has been exposed to eCO2, +20% relative to ambient concentrations (aCO2), for 15 years. We applied in the field an ammonium-nitrate fertilizer solution, in which either ammonium (NH4+) or nitrate (NO3-) was labelled with 15N. The simultaneous gross N transformation rates were analysed with a 15N tracing model and a solver method. The results confirmed that after 15 years of eCO2 the N2O emissions under eCO2 were still more than 2-fold higher than under aCO2. The tracing model results indicated that plant uptake of NH4+ did not differ between treatments, but uptake of NO3- was significantly reduced under eCO2. However, the ratio of gross production and consumption of NH4+ remained unchanged under eCO2, but decreased slightly for NO3-, which increased NO3- availability under eCO2. The N2O isotopic signature indicated that under eCO2 the sources of the additional emissions, 8407 µg N2O-N m-2 during the first 58 days after labelling, were associated with NO3- reduction (+2.0%), NH4+ oxidation (+11.1%) and organic N oxidation (+86.9%). We presume that increased root exudation under eCO2 provided an additional source of bioavailable supply of energy that triggered the stimulation of microbial soil organic matter (SOM) mineralization, as a priming effect, and an increased activity of bacterial nitrite reductase, which caused the shift in N2O:N2 emission ratio, via incomplete denitrification, explaining the positive feedback reaction of doubled N2O emissions.

Abstract:

Rising atmospheric CO2 concentrations are expected to increase nitrous oxide (N2O) emissions from soils via changes in microbial nitrogen (N) transformations. Several studies have shown that N2O emission increases under elevated atmospheric CO2 (eCO2), but the underlying processes are not yet fully understood. Here, we present results showing changes in soil N transformation dynamics from the Giessen Free Air CO2 Enrichment (GiFACE): a permanent grassland that has been exposed to eCO2, +20% relative to ambient concentrations (aCO2), for 15 years. We applied in the field an ammonium-nitrate fertilizer solution, in which either ammonium (NHþ 4 ) or nitrate (NO 3 ) was labelled with 15N. The simultaneous gross N transformation rates were analysed with a 15N tracing model and a solver method. The results confirmed that after 15 years of eCO2 the N2O emissions under eCO2 were still more than twofold higher than under aCO2. The tracing model results indicated that plant uptake of NHþ 4 did not differ between treatments, but uptake of NO 3 was significantly reduced under eCO2. However, the NHþ 4 and NO 3 availability increased slightly under eCO2. The N2O isotopic signature indicated that under eCO2 the sources of the additional emissions, 8,407 lg N2O–N/m2 during the first 58 days after labelling, were associated with NO 3 reduction (+2.0%), NHþ 4 oxidation (+11.1%) and organic N oxidation (+86.9%). We presume that increased plant growth and root exudation under eCO2 provided an additional source of bioavailable supply of energy that triggered as a priming effect the stimulation of microbial soil organic matter (SOM) mineralization and fostered the activity of the bacterial nitrite reductase. The resulting increase in incomplete denitrification and therefore an increased N2O:N2 emission ratio, explains the doubling of N2O emissions. If this occurs over a wide area of grasslands in the future, this positive feedback reaction may significantly accelerate climate change.